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  Optimization of a PCR Method for Fingerprinting Potato Varieties

Xiu-Qing Li1, Shirlyn Coleman2, Birt Stevens1 and Muhammad Haroon1
(Partner: Potato Research Centre, Agriculture and Agri-Food Canada)

Abstract: An optimized protocol for fingerprinting potato varieties with SSR-PCR (PCR of the microsatellite DNA region) for the patatin gene was developed using appropriate reaction conditions (7 °C lower than Tm temperature). The optimized method can unambiguously distinguish the three potato varieties Shepody, Russet Burbank, and Niska tested whereas the widely used unoptimized method could not.

1Potato Research Centre, Agriculture and Agri-Food Canada, 850 Lincoln Road, P.O. Box 20280, Fredericton, NB E3B 4Z7
2Plant Propagation Centre, NBDARD, Fredericton, NB E3B 5H1

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